Advances in Research on Diabetes and Ocular Surface Demodex
Li Zhenghao, Su Yang, Li Siqi, Yi Guoguo*, Fu Min#
Zhujiang Hospital of Southern Medical University, Guangzhou, China
Demodex infection on ocular surface is a common ocular parasitic diseaseï¼ which harms patients' physical and mental health and quality of life. Studies show that the incidence of ocular Demodex in diabetic patients is higher than that in normal people, and as the incidence of diabetes increases year by year, the ocular Demodex infection also increases, making it a disease that impairs the normal function of the eye of diabetic patients. However, the mechanism of diabetic ocular surface Demodex infection is still unclear and the treatment method is single and lacking. And there is no unified standard and lack of clinical data on the prognosis of patients. It needs more concern of patients and even endocrinologists and ophthalmologists. This article summarizes and analyzes the recent studies on diabetes and ocular surface Demodex infection in order to provide reference for clinical diagnosis and treatment.
The infection rate of ocular surface Demodex in diabetic patients is higher than that in normal people according to previous studies1. This indicates that there may be a correlation between ocular mite infection and diabetes. Based on the IDF Diabetes Atlas, global estimate of diabetes prevalence over 18 years old was 8.8%. These numbers are expected to increase to 9.9% in 20452. Diabetes mellitus has become one of the top chronic diseases that threaten the health of our citizens. With the increase of diabetes, the associated ocular surface Demodex infection rate may also increase, which further increases the difficulty in treating ocular surface Demodex and threaten the eye health of diabetic patients.
As the relevant studies are rare and the mechanism of ocular epidemic Demodex infection in diabetes mellitus is unclear, this article summarizes and analyzes the recent studies on diabetes mellitus and ocular surface Demodex infection, in order to provide a reference for clinical diagnosis and treatment and further mechanism exploration.
Overview of Ocular Surface Demodex Infection
Demodex and ocular infection
Demodex is a small permanent parasitic mite parasitizing in human and mammalian hair follicles and sebaceous glands3. There are two types of Demodex that can reside in the human body, namely Demodex folliculorum and Demodex brevis4,5. In the eyes, Demodex folliculorum usually accumulate in clusters in the roots of the eyelashes and follicles, causing frontal blepharitis. Demodex brevis live alone in sebaceous glands and tarsal glands, causing posterior blepharitis6.
According to recent studies, Demodex infection is associated with many common ocular surface diseases, such as adult blepharitis7,8 and allergic conjunctivitis9. Patients with ocular surface Demodex infection often have clinical symptoms such as dryness, burning, grittiness, photaesthesia and trichiasis. Blurred vision and decreased vision may occur when the cornea is involved. Demodex infection can occur in all ages. The onset of Demodex infection is usually slow and eyes are generally both affected.
Diagnosis of ocular surface demodex
1. Chronic or subacute course of disease in both eyes, with ocular symptoms such as redness, itching and foreign body sensation, or accompanied by repeated attacks and refractory chalazel swelling.
2. Abnormal eyelashes, lipid sleeve secretions at the root of eyelashes (with diagnostic value), and hyperemia and hypertrophy at the edge of eyelid.
3. Positive for Demodex mite test. (1) Demodex mites in all stages were counted; (2) in adult patients, the count of Demodex in any one of the four eyelids reached 3 /3 eyelashes; (3) if it is less than the above criteria, it is suspicious and positive, and it should be combined with clinical manifestations. If necessary, it can also be checked for other pathogenic microorganisms, such as bacteria and fungi.
At the same time in line with the above 3, it can confirm the Demodex palpebrachitis. In line with the first two, it can be repeatedly taken and examined with the help of laser confocal microscope in vivo in who Demodex mites were detected in eyelashes but the count was not up to the standard. If the count is still not up to the standard, it can be diagnosed as suspected Demodex palpebrachitis. Demodex palpebrachitis was not diagnosed if only Demodex was positive without clinical signs and symptoms10.
Treatment of ocular surface demodex infection
At present, the clinical treatment of ocular surface Demodex infection is not multiple. 2% mercuric chloride can be used to alleviate the infection of ocular surface Demodex, while some patients have been reported to have toxic effects after application11. Demodex can be eradicated in 4 weeks after the use of tea tree essential oil12. However, contact dermatitis is prone to occur when using low concentration tea tree essential oil locally13. Besides it has high cost and long treatment cycle. In addition, tobramycin dexamethasone eye ointment also has a good therapeutic effect on ocular surface Demodex14. After Junk et al. used eyelid wash solution combined with topical 2% metronidazole gel for a month, chronic otitis parenchyma was relieved and the number of ocular surface Demodex was reduced by half15. Oral ivermectin can also treat and alleviate ocular surface Demodex infection16, and combination therapy between ivermectin and metronidazole(71.6% complete remission) was better than ivermectin alone(45% complete remission )17. At present, there is no clinical data on the recurrence and prognosis of ocular surface Demodex infection around the world.
Clinical Study on Diabetes Mellitus and Ocular Surface Demodex Infection
Diabetes mellitus is a group of metabolic diseases characterized by chronic hyperglycemia caused by multiple causes which caused by the deficiency of insulin secretion and/or action. With the increasing prevalence of diabetes, the incidence of eye diseases related to diabetes is also increasing year by year.
According to relevant studies, the infection rate of ocular surface Demodex in diabetic patients is much higher than that in normal people. As early as 1990, Clifford CW et al. reported that diabetic patients had an increased risk of ocular surface Demodex infection compared with normal people18. In 2017, Zeynep Tas Cengiz et al. analyzed the potential pathogenic factors of ocular surface Demodex infection and found that in diabetic patients, ocular surface Demodex infection rate was higher than that in healthy controls19. Poor glycemic control in diabetic patients also increases the risk of ocular surface Demodex infection. In Gökçe Cumali et al. 's study, people with poor glycemic control (HbA1c>7%) had a higher infection rate of ocular surface Demodex than those with good glycemic control (HbA1c≤7%)20. This suggests that poor glycemic control may increase the susceptibility of diabetic patients to Demodex. The study also pointed out that the higher the blood sugar level of diabetic patients, the higher the risk of ocular surface Demodex infection20. It suggests that the infection of eye Demodex may be related to the blood glucose concentration in diabetic patients, and diabetic patients with eye diseases will further increase the incidence of eye Demodex infection. In a 2011 study of 84 samples (42 normal subjects and 42 diabetic retinopathy patients), the odds of detecting ocular surface Demodex were greater in the diabetic retinopathy group than in the control group21. The above studies indicate that diabetic patients have a greater susceptibility to ocular surface Demodex and are more likely to cause the infection of ocular surface Demodex, which not only further increases the difficulty of treating ocular surface Demodex, but also further poses a threat to the physical and mental health of diabetic patients.
Ocular Demodex infection, a common complication of diabetes, will further threaten the eye health and normal eye function of these patients and endanger the physical and mental health of patients. Some cross-sectional studies on diabetes mellitus and ocular surface Demodex infection indicate that diabetes mellitus can increase the infection rate of ocular surface Demodex. However, prospective studies on diabetes mellitus complicated with ocular surface Demodex are still insufficient. There are not enough studies on the clinical diagnosis, clinical treatment, pathogenic mechanism and patient prognosis of diabetes associated with ocular Demodex. More prospective clinical studies are needed to provide evidence-based evidence for the causal relationship between the two, so as to provide a reference for the prevention and clinical treatment of diabetes associated with ocular Demodex.
Pathogenic Factors of Increased Ocular Surface Demodex Infection Caused by Diabetes Mellitus
Studies have shown that the infection rate of Demodex increases in patients with chronic renal failure19, HIV, undergoing hemodialysis22, cancer chemotherapy23 and other weakened immunity. In patients with diabetes, many studies have shown that the body immunity of patients with diabetes will be reduced to different degrees due to various reasons, such as neutrophil chemotaxis24, mast cell function25, the interaction between white blood cells and endothelial cells26, lymph node clearance of pathogens27 decreased, TNF, IL and prostaglandin release decreased28. The physiological dysfunction of these cells or cytokines will lead to the decrease of immune function of the body and increase the susceptibility to ocular Demodex. In normal healthy people, when the Demodex is in excessive proliferation, the strong immunity will cause a series of immune reactions in the body and inhibit the proliferation of the Demodex in order to resist the damage of the Demodex to the ocular surface. However, when the immunity of the body is decreased and the immune function is low, it can not effectively resist the infection of eye Demodex and cause a series of related inflammatory reactions. Therefore, the decrease of immunity may be an important reason for the increase of ocular Demodex infection rate in diabetic patients.
Changes of ocular surface flora
There is normal bacterial flora on the ocular surface constituting a stable microbial community on the ocular surface and participating in regulating the normal micro-environment of the ocular surface in the normal ocular surface. Y. Huang et al. used 16s-rDNA gene sequencing to detect that the core flora of conjunctival microbial community consisted of Corynebacterium, Pseudomonas, Staphylococcus, Acinetobacter, Streptococcus, Micrococcus, Anaerobic bacteria, ciliates, Siemens and Micrococcus29. In patients with diabetes mellitus, normal ocular flora changes due to elevated blood sugar, decreased immunity, microvascular changes and so on. In patients with diabetes, the numbers of staphylococcus epidermidis30 , staphylococcus aureus Acinetobacter and Pseudomonas at the genus level31 were higher than normal, and the numbers of gram-negative cocci, escherichia coli, klebsiella pneumoniae32 and Bacteroidetes31 were also slightly higher than normal. Proteobacteria were at a lower abundance31. Changes in normal flora of ocular surface in diabetic patients lead to disturbance of ocular surface microecology.
In a normal organism, ocular Demodex are commonly found in the head and face33, which can regulate the activities of ocular surface bacteria and participate in maintaining the balance of ocular surface microecology. Relevant studies have shown that the symptoms of the eyes become worse after anti-mite treatment and then combined anti-bacterial treatment can make the disease relieve, which indicates that there is a balance between Demodex and ocular surface bacteria34. R Wolf et al. showed that Demodex could carry large amounts of bacteria on their surfaces, such as streptococcus and staphylococcus. The increase of Demodex proliferation makes the normal flora of ocular surface change, which causes the change of ocular surface microenvironment35. The increase of staphylococcus epidermidis and staphylococcus aureus in the ocular surface of diabetic patients may lead to the corresponding increase in the number of bacteria on the surface of eye Demodex, thereby increasing the probability of bacteria causing ocular surface diseases and increasing the occurrence of an inflammation on the ocular surface.
Changes of hair follicles and sebaceous glands
The hyperglycemia environment in the blood of patients with diabetes will damage the endothelial cells of blood vessels in the whole body, leading to different degrees of lesions of blood vessels in the whole body, especially the small arteries in the whole body are prone to lesions36. The supply of oxygen and nutrients to cells in normal tissues and organs is completed by the corresponding arteries. However, due to the pathological changes in the blood vessels of patients with diabetes, tissues and organs supplied will be damaged to varying degrees, eventually leading to complications of diabetes37. Hair follicles are divided into terminal hair follicles, sebaceous glands, and hair follicles38,39. Most of the terminal hair follicles and sebaceous glands are located on the ocular surface. Hair follicles are active organs in the body, which need a special microenvironment, such as sufficient oxygen and nutrition. They are easy to be damaged during the process of diabetes, leading to hairless, fragile and slow growth40,41. Hair follicles remain empty for a period of time after hair loss and microorganisms are prone to invade hair follicles resulting in an infection of the hair follicles42,43. Also, in diabetes, it causes follicular dilation and changes in lipid synthesis44. The Demodex infection will lead to sebaceous gland secretion disorder, leading to increased sebum secretion45. Sebum increase will accelerate the proliferation of Demodex as Demodex habit to feed on sebum46. The infection of ocular Demodex in diabetic patients may increase due to the damage of hair follicles, the change of the physiological state of hair follicle sebaceous glands, the increase of lipid synthesis, the hair follicle emptiness caused by hair loss and other factors.
At present, the mechanism of the increase of diabetic ocular Demodex infection is not clear, and further research is needed.
Summary and Prospect
With the increasing number of diabetic patients, the incidence of ocular surface diseases in diabetic patients increases year by year. It is indicated above that there is a correlation between ocular Demodex infection and diabetes. It is important to prevent and control ocular surface Demodex infection in diabetic patients, improve the symptoms of ocular surface Demodex infection, and effectively treat ocular surface Demodex infection, so as to improve the quality of life of diabetic patients.
When patients with diabetes experience redness, itching and foreign body sensation, or are accompanied by recurrent attacks and chalazel swelling, doctors should consider whether there is ocular Demodex infection and improve relevant examinations if other diseases cannot be ruled out. Now ocular Demodex can be treated with 2% mercuric chloride, tea tree essential oil, tobramycin dexamethasone eye ointment, topical 2% metronidazole gel, Oral ivermectin and so on. Because of the change of ocular surface flora in diabetic patients, the treatment of ocular surface Demodex should be combined with antibacterial treatment to relieve symptoms.
At present, due to the single treatment means, the majority of medical workers are still required to further clinical and basic research on ocular surface Demodex infection. It is not clear why Ocular surface caused by diabetes Demodex infection rate increased and how to treat the patients. We still need more prospective clinical study of the causal relationship between them to provide evidence-based study and more basic research based on both the pathophysiology of correlation for further elaboration, in order to provide a reference for clinical treatment.
- Zhang Yu. Clinical study on the effect of demodex mite on ocular surface function in patients with type 2 diabetes. D Southern medical university. 2019.
- Cho NH, Shaw JE, Karuranga S, et al. IDF Diabetes Atlas: Global estimates of diabetes prevalence for 2017 and projections for 2045. J Diabetes research and clinical practice.2018.
- Ferrer L, Ravera I, Silbermayr K. Immunology and pathogenesis of canine demodicosis. J Veterinary Dermatology. 2014; 25(5): 427-465.
- Rufli T, Mumcuoglu Y. The hair follicle mites Demodex folliculorum and Demodex brevis: biology and medical importance. A review J Dermatology. 1981; 162(1): 1-11.
- Nicholls SG, Oakley CL, Tan A, et al. Demodex species in human ocular disease: new clinicopathological aspects. J Int Ophthalmol. 2017; 37(1): 303-312.
- Elston CA, Elston DM. Demodex mites. J Clinics in Dermatology. 2014; 32(6): 739-743.
- Bhandari V, Reddy JK. Blepharitis: always remember demodex. J Middle East African Journal of Ophthalmology. 2014; 21(4): 317.
- Yingying Gao, Duanlian Xu, Lijuan Huang, et al. Clinical analysis of the relationship between the scaly ridges and the orbital Demodex. J Chinese Journal of Ophthalmology. 2009; 45(9): 834-835.
- Kim JT, Lee SH, Chun YS, et al. Tear cytokines and chemokines in patients with Demodex blepharitis. J Cytokine. 2011; 53(1): 94-99.
- Chinese branch of Asian dry eye association, ophthalmology group of ophthalmologycommittee of cross-strait medical exchange association. Consensus of diagnosis and treatment experts on vermicoid mite blepharitis in China 2018. J Chinese journal of ophthalmology. 2018; 54(7): 491-495.
- Rodríguez AE, Ferrer C, Alió JL. [Chronic blepharitis and Demodex. J Archivos De La Sociedad Espanola De Oftalmologia. 2005; 80(11): 635.
- Gao YY, Di Pascuale MA, Li W, et al. In vitro and in vivo killing of ocular Demodex by tea tree oil. J British Journal of Ophthalmology. 2005; 89(89): 1468-1473.
- Halcón L, Milkus K. Staphylococcus aureus and wounds: a review of tea tree oil as a promising antimicrobial. J American Journal of Infection Control. 2004; 32(7): 402-408.
- Ke Lan, Yang Yanning, Huang Linying, et al. Observation of the treatment of demodex blepharitis with tobramycin dexamethasone eye ointment combined with tea tree oil. J Chinese Journal of Optometry Ophthalmology and Visual Science. 2017; 19(3): 176-181.
- Junk AK, Lukacs A, Kampik A. [Topical administration of metronidazole gel as an effective therapy alternative in chronic Demodex blepharitis--a case report. J Klinische Monatsblatter fur Augenheilkunde. 1998; 213(1): 48-50.
- Holzchuh FG, Hida RY, Moscovici BK, et al. Clinical treatment of ocular Demodex folliculorum by systemic ivermectin. J American Journal of Ophthalmology. 2011; 151(6): 1030-1034.
- Salem DA, El-Shazly A, Nabih N, et al. Evaluation of the efficacy of oral ivermectin in comparison with ivermectin-metronidazole combined therapy in the treatment of ocular and skin lesions of Demodex folliculorum. J International Journal of Infectious Diseases Ijid Official Publication of the International Society for Infectious Diseases. 2013; 17(5): e343.
- Clifford CW, Fulk GW. Association of Diabetes, Lash Loss, and Staphylococcus aureus with Infestation of Eyelids by Demodex folliculorum Acari Demodicidae. J Journal of Medical Entomology. 1990; 27(4): 467-470.
- Zeynep Tas Cengiz,Hatice Uce Ozkol,Yunus Emre Beyhan,Murat Ozturk,Hasan Yilmaz. Evaluation of some chronical diseases in etiopathogenesis of demodicosis. J Dermatologica Sinica. 2017.
- Gökçe C, Aycan-Kaya Ö, Yula E, et al. The effect of blood glucose regulation on the presence of opportunistic Demodex folliculorum mites in patients with type 2 diabetes mellitus. J J Int Med Res. 2013; 41(5): 1752-1758.
- Yamashita LS, Cariello AJ, Geha NM, et al. Demodex folliculorum on the eyelash follicule of diabetic patients. J Arquivos Brasileiros De Oftalmologia. 2011; 74(6): 422-424.
- Yagdiran DO, Aytekin S. Comparison of Demodex folliculorum density in haemodialysis patients with a control group. J Journal of the European Academy of Dermatology & Venereology Jeadv. 2007; 21(4): 480.
- Nara T, Katoh N, Inoue K, et al. Eosinophilic folliculitis with a Demodex folliculorum infestation successfully treated with ivermectin in a man infected with human immunodeficiency virus. J Clinical & Experimental Dermatology. 2009; 34(8): e981-e983.
- Chang HN. Chemotaxis of blood monocytes and polymorphonuclear leukocytes from patients with diabetes mellitus. J Zhonghua Minguo wei sheng wu xue za zhi Chinese journal of microbiology. 1979; 12(4): 136-139.
- Bagdade JD, Nielson KL, Bulger RJ. Reversible abnormalities in phagocytic function in poorly controlled diabetic patients. J American Journal of the Medical Sciences. 1972; 263(6): 451-456.
- Sannomiya P, Oliveira MA, Fortes ZB. Aminoguanidine and the prevention of leukocyte dysfunction in diabetes mellitus: a direct vital microscopic study. J British Journal of Pharmacology. 1997; 122(5): 894-898.
- Moriguchi P, Sannomiya P, Lara PF, et al. Lymphatic system changes in diabetes mellitus: role of insulin and hyperglycemia. J Diabetes/metabolism Research & Reviews. 2005; 21(2): 150-157.
- Alba-Loureiro TC, Martins EF, Landgraf RG, et al. Role of insulin on PGE 2 generation during LPS-induced lung inflammation in rats. J Life Sciences. 2006; 78(6): 578-585.
- Huang Y, Yang B, Li W. Defining the normal "core microbiome" of conjunctival microbialcommunities. J Clinical Microbiology & Infection the Official Publication of the EuropeanSociety of Clinical Microbiology & Infectious Diseases. 2016; 22(7): 643-647.
- Martins EN1, Alvarenga LS, Höfling-Lima AL, et al. Aerobic bacterial conjunctival flora in diabetic patients. J Cornea. 2004; 23(2): 136-142.
- Li Siqi, Yi Guoguo, Peng Hui,et al. How Ocular Surface Microbiota Debuts in Type 2 Diabetes Mellitus. J Frontiers in cellular and infection microbiology. 2019; 9.
- Fernández-Rubio ME, Rebolledo-Lara L, Martinez-García M, et al. The conjunctival bacterial pattern of diabetics undergoing cataract surgery. J Eye. 2010; 24(5): 825.
- Thoemmes MS, Fergus DJ, Urban J, et al. Ubiquity and diversity of human-associated Demodex mites. J Plos One. 2014; 9(8): e106265.
- Nicholls SG, Oakley CL, Tan A, et al. Demodex species in human ocular disease: new clinicopathological aspects. J Int Ophthalmol. 2017; 37(1): 303-312.
- Wolf R, Ophir J, Avigad J, et al.The hair follicle mites Demodex spp Could they be vectors of pathogenic microorganisms? J Acta dermato-venereologica. 1988; 68(6): 535-537.
- Stephenson J, Fuller JH. Microvascular and acute complications in IDDM patients the EURODIAB IDDM Complications Study. J Diabetologia. 1994; 37(3): 278-285.
- Pambianco G, Costacou T, Ellis D, et al. The 30-year natural history of type 1 diabetes complications: the Pittsburgh Epidemiology of Diabetes Complications Study experience. J Diabetes. 2006; 55(5): 1463.
- Sperling LC. Hair anatomy for the clinician. J Journal of the American Academy of Dermatology. 1991; 25(1 Pt 1): 1-17.
- Piérard GE, Piérard-Franchimont C, Marks R, et al. EEMCO group European Expert Group on Efficacy Measurement of Cosmetics and other Topical Products. EEMCO guidance for the assessment of hair shedding and alopecia. J Skin Pharmacol Physiol. 2004; 17(2): 98-110.
- Plikus Maksim V, Van Spyk Elyse N, Pham Kim, et al. The circadian clock in skin: implications for adult stem cells, tissue regeneration, cancer, aging, and immunity. J Journal of Biological Rhythms. 2015; 30(3): 163.
- Hayashi S, Miyamoto I, Takeda K. Measurement of human hair growth by optical microscopy and image analysis. J British Journal of Dermatology. 1991; 125(2): 123.
- Andl T, Ahn K, Kairo A, et al. Epithelial Bmpr1a regulates differentiation and proliferation in postnatal hair follicles and is essential for tooth development. J Development. 2004; 131(10): 2257-2268.
- Paus R, Christoph T, Müllerröver S. Immunology of the hair follicle: a short journey into terra incognita. J J Investig Dermatol Symp Proc. 1999; 4(3): 226-234.
- Cakmak S, Gül U, Gönül M, et al. Statin therapy and diabetic skin. J Advances in Therapy. 2008; 25(1): 17-22.
- Diaz-Perez JL. Demodex mites in rosacea. J Journal of the American Academy of Dermatology. 1994; 30(5): 812-813.
- Farrant S. Demodex -- at the root of the problem. J Optometry Today. 2014.